Practical Course: Single Cell Gene Expression Analysis

Country: Germany

City: Heidelberg

Abstr. due: 23.01.2015

Dates: 17.04.15 — 22.04.15

Area Of Sciences: Biology;

Organizing comittee e-mail:

Organizers: EMBL


The analysis of transcripts in minute samples is often used to increase the knowledge in a plethora of research fields. Precise sampling is crucial for the success of the approach and depends on the nature of the sample and the goal of the experiment. This course will present flow cytometry, Fluidigm and laser capture microdissection as methods to obtain single cells for the subsequent gene expression analysis, which cannot be performed by conventional RNA methods due to their low sensitivity. This course will present different techniques to assess gene expression in individual cells, including RT-qPCR, Next Generation Sequencing and Proximity Extension Assay.  Data collected by these methods will be analysed in the very crucial session of data analysis, which constitutes another strong component of the course.

This course targets scientists who are entering the field of single cell analysis and want to learn about different preparation methods and gene expression profiling methods. The participants will learn about the complete workflow and gain understanding about the possibilities and limitations of the approach. The course will cover lectures and hands-on sessions by experts in the fields and is aimed for PhD students, postdoctoral fellows or other scientists with a solid qPCR and molecular biology background.

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