Postdoc Position at University of Basel

Country: Switzerland;

City: Basel

Vacancy added: 06.11.2018

Employer: University of Basel

Vacancy type: PostDoc vacancy;

 

The Division of Molecular and Systems Toxicology, Department of Pharmaceutical Sciences, is offering a Postdoc position in LC-MS/MS analytics to investigate mechanisms involved in steroid – oxysterol – bile acid homeostasis.

Your position
This project focuses on the role of the endoplasmic reticulum (ER) enzymes 11β-hydroxysteroid dehydrogenase 1 (11βHSD1) and hexose-6-phosphate dehydrogenase (H6PDH) in the metabolism of steroids, oxysterols and bile acids, with relevance to inflammatory and metabolic diseases. Within the ER, 11βHSD1 forms a complex with H6PDH that provides cofactor NADPH. 11βHSD1 is considered a therapeutic target in metabolic and immune disorders. The function of both 11βHSD1 and H6PDH and especially their impact on oxysterol and bile acid homeostasis needs to be further explored, in order to understand metabolic diseases and potentially contributing to diagnostic and therapeutic applications.

The successful candidate should have experience in the application and development of UPLC-MS/MS based analytical methods, ideally for the quantification of endogenous small molecules. She/he should also have experience in sample preparation. Additionally, the candidate will be responsible in part for the maintenance of the instrument. The projects will include quantification of analytes in samples from human/animals, tissues, cell culture supernatants, and cell lysates or microsomal preparations. Whilst most of the methods are established to continue ongoing studies, the successful candidate is expected to further develop and validate analytical methods to contribute with her/his expertise to projects of other team members and external collaboration partners. In collaboration with team members, UPLC-MS/MS will be employed to investigate the role of 11βHSD1 and H6PDH on steroid, oxysterol and bile acid profiles in mouse models (H6PD KO; pharmacological inhibition).

The successful candidate will be integrated in an international research group. We employ a multi-disciplinary approach and look for a highly motivated, interactive team player.

Your profile

  • PhD in the field of Life Sciences
  • Experience in LC-MS/MS quantification of small molecules
  • Experience with sample preparation (extraction from animal/human tissues, culture supernatants, enzyme assays using lysates and microsomal preparations)
  • Knowledge in the analysis of samples from metabolomics studies
  • Ideally, basic knowledge and skills in animal experimentation
  • Experience in supervision of students
  • Fluent communication in English, written and oral, ideally good knowledge in German
  • Highly motivated, interactive team player


We offer you

  • Postdoc position for two years (with possibility of extension)
  • Training into the key techniques in order to take over ongoing projects
  • International research environment


Application / Contact
Applications should include CV, a short motivation letter, copies of degrees and qualifications and the names/contact data of at least two referees. Documents should be submitted electronically to Christiane.Kocher@unibas.ch.
Deadline: November 30, 2018

 
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