Biomolecular interaction analysis 2016: From molecules to cells

Страна: Португалия

Город: Porto

Тезисы до: 20.07.2016

Даты: 07.11.16 — 11.11.16

Е-мейл Оргкомитета:

Организаторы: EMBO


A comprehensive and integrated characterization of molecular interactions is key to the understanding of life processes at a molecular level. Due to their complexity the study of binding events requires orthogonal approaches, i.e. the gathering of data from multiple methods in order to build a complete picture of their function.

Hence this practical course aims at a multidisplinary approach to the study of molecular interactions from and in biological systems, integrating biochemical/biophysical methodologies with live microscopy techniques.

In this 3rd Edition the course will cover a range of methods used in the kinetics and thermodynamics characterization of binding events, such as:

    Isothermal Titration Calorimetry (ITC)
    Surface Plasmon Resonance (SPR)
    Microscale Thermophoresis (MST)
    Surface Acoustic Wave (SAW)
    Electro-Switchable Biosurfaces (switchSENSE)

The bridge to in vivo will be crossed with the introduction of advanced light microscopy techniques, e.g.:

    Fluorescence Resonance Energy Transfer (FRET)
    Fluorescence Lifetime Imaging Microscopy (FLIM)

to study the dynamic interactions of proteins within the living cell.

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