Double immobilization of enzymes

Scheme of hybrid material structure

Schemes of hybrid materials synthesis: Formation of silica components in composites (1) through the sol-gel process and (2) by means of fumed silica A300 with nanoparticle aggregates

Microphotographs of two types of hybrid materials obtained using scanning electron microscopy (SEM). Formation of silica components in composites (A) through the sol-gel process and (B) by means of A300 with nanoparticle aggregates.

Immobilization of enzymes by inclusion into a polymeric shell provides effect similar to one in vivo, but, unfortunately, polymers are unaligned to bactericidal influence. The use of hybrid organic-inorganic composites gives an additional advantage caused by double protection of enzymatic preparation from negative effects of environment.

The grounds of crating of such systems linked to factors affected  their formation, as well as subsequent development, will allow us to obtain materials with requirement characteristics. Previously, it was shown that pore structure of silica used was slightly dependent on the nature of a polymer in a composite, as well as on the pH value of sol-gel medium.

Polymer shells:

- polyvinyl alcohol, PVA with molecular mass of 40000 and 95000;

- gelatine with molecular mass of 10800 and 46500;

- polymeric composition based on PVA  and polyacrylic acid (PAA)  (PAA:PVA = 10:1, pH  6).

Developer:

Chuiko Institute of Surface Chemistry (CISC) of National Academy of Sciences of Ukraine

Contacts: 
Kyiv, Ukraine: Tel.: + 38 (044) 422 96 04; Fax: + 38(044) 424 35 67; e-mail: [email protected]