Postdoctoral position (2 years) on chemo-optogenetics [closing 16.02.2021]

Страна: Швеция;

Город: Umeå

Добавлена: 25.01.2021

Работодатель: Umeå University

Тип: PostDoc vacancy;

Для кого: For researchers;

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Дедлайн подачи: 16.02.2021

 

Umeå University offers exciting research opportunities in an excellent environment. The Department of Chemistry has around 200 employees. The Department is located within the cross-disciplinary Chemical Biological Centre (video) (https://www.umu.se/en/chemical-biological-centre/) at Umeå University and provides excellent facilities and state-of-art equipment and platforms in a creative, inspiring, international and highly interactive environment. Facilities include Protein Expertise Platform, X-ray, Proteomics, NMR (850-400 MHz), Cryo-EM and Biochemical Imaging Centre (confocal, FLIM, spinning disk, TIRF, STORM).

The recent breakthrough research from Umeå University include deciphering the molecular mechanisms of the bacterial CRISPR-Cas9 system and repurposing it into a tool for genome editing, a method developed at Umeå university that was awarded Nobel Prize in Chemistry 2020.

Project description and working tasks
Genetic perturbations by gain or loss of gene function through overexpression, knock-out or knock-down approaches is powerful for biological studies. However, traditional genetic approaches are chronic (hours to days). Consequently, the phenotype may not be detected due to adaptation and the dynamics of phenotypic change cannot be followed.

Chemical/light-inducible approaches provide means to control biological systems with spatial and temporal resolution that is unmatched by traditional genetic perturbations. However, canonical optogenetic systems using photoreceptors suffer from inherent limitations in basal activity in the dark, dynamic range and maneuverability.

Our laboratory has pioneered in the development of chemo-optogenetic systems (Chem 2019 review). We have developed the first bioorthogonal and reversible chemically induced dimerization (CID) system (SLF'-TMP system) for controlling protein function by small molecules in live cells (Angew Chem 2014). A photoactivatable CID (pCID) using photo-caged dimerizer (NovcTMP-Cl) (Angew Chem 2017) and photo-switchable CID system (psCID) using photo-switchable dimerizer (CONC) allow superior spatiotemporal control of protein function in live cells (Angew Chem 2018). We have developed novel "Molecular Activity Painting (MAP)" strategy by combining pCID with immobilized artificial receptors to enable "painting" of signaling molecules and their activity at micrometer-scale at the plasma membrane. Using photoactivatable dual-chemically induced dimerization (pdCID) system, we developed a Multi-directional Activity Control (MAC) approach to spatiotemporally control cellular signaling and intracellular cargo transport (Angew Chem 2018). This project will focus on development of chemo-optogenetic systems, which enable the activity to be controlled by light with high spatial and temporal resolution in live cells and organisms. The techniques used in the project include protein/peptide synthesis, organic synthesis and cell imaging.

The project is funded by European Research Council (ERC) and the Knut and Alice Wallenberg Foundation (KAW). The project is interdisciplinary with strong collaborations across scientific disciplines. The position is full-time in two years with starting date as soon as possible, or as agreed.

 
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